Thymosin beta-4, also known as Thymosin β4, Tβ4, TB4, TB500 (refers to synthetic fragment Ac-LKKTETQ), is a 43-amino acid research single peptide. CAS Number: 77591-33-4. Molecular weight: 4963.55 Da. Sequence: SDKPDMAEI EKFDKSKLKK TETQEKNPLP SKETIEQEKQ AGES (43 amino acids).

Wound healing and tissue repair (Ann N Y Acad Sci); cardiac protection post-MI via ErbB2 signaling (Cardiovasc Res); anti-fibrotic effects in pulmonary fibrosis (J Pharm Pharmacol); fat graft survival via mitochondrial transfer (Free Radic Biol Med); neuroprotection in Alzheimer’s and prion models (Stem Cell Reports, Molecules) All findings referenced are from preclinical and in vitro studies. No approved clinical applications exist for this research compound.

-20°C or -80°C, desiccated, protected from light Avoid repeated freeze-thaw cycles. Once reconstituted, the solution should be refrigerated at 2-8°C and used within 28 days.

Every batch of Thymosin Beta 4 from Peptide.co undergoes independent third-party HPLC testing. Current verified batches: 5mg batch (Lot #YPB.214): 99.9% purity; 10mg batch (Lot #YPB.215): 99.8% purity. Full Certificates of Analysis are publicly available at coa.peptide.co, covering HPLC purity, mass spectrometry identity confirmation, heavy metals screening (<20 ppb), and microbial limits (TAMC/TYMC: 0 CFU). All products are manufactured in FDA-registered facilities in the United States.

Actin-sequestering protein regulating actin polymerization, cell migration, differentiation, and proliferation; modulates ErbB2/Raf1, JAK2/STAT3, TGF-β pathways; anti-inflammatory and anti-apoptotic effects These mechanisms have been characterized in preclinical models only.

Thymosin Beta 4 is supplied as a lyophilized (freeze-dried) powder and must be reconstituted before use in research applications. The standard reconstitution solvent is bacteriostatic water (sterile water containing 0.9% benzyl alcohol). When reconstituting, direct the solvent gently along the inside wall of the vial — do not spray directly onto the lyophilized powder. Allow the peptide to dissolve naturally without shaking or vortexing. For detailed reconstitution volume calculations, use the free Bacteriostatic Water Calculator or Peptide Reconstitution Calculator on Peptide.co.