PNC-27 is a synthetic chimeric peptide consisting of a p53 protein fragment (residues 12-26, the HDM-2 binding domain) fused to a cell-penetrating membrane-active sequence. Molecular weight: approximately 3000 Da. It has been studied in preclinical research for its selective interaction with HDM-2 protein overexpressed on the surface of certain transformed cell lines.

Preclinical studies in cancer cell lines including pancreatic (MIA PaCa-2, TUC-3), breast (MCF-7), melanoma (A-2058), leukemia, primary tumors; nude mouse xenograft models; visualized pores via immuno-electron microscopy; effective against chemotherapy-resistant cells (PNAS 2010, Biomedicines 2022) All findings referenced are from preclinical and in vitro studies. No approved clinical applications exist for this research compound.

Lyophilized powder: -20°C short-term (weeks-months) or preferably -80°C long-term; avoid light, freeze-thaw cycles, pH >8 (Sigma-Aldrich guidelines, GenScript) Once reconstituted, the solution should be refrigerated at 2-8°C and used within 28 days.

Peptide.co’s PNC-27 has been verified at 99.1% purity through independent third-party HPLC testing. The current batch (Lot #YPB.275, 10mg) includes a full Certificate of Analysis confirming identity, purity, and peptide content. All COAs are publicly available at coa.peptide.co. Testing covers HPLC purity analysis, mass spectrometry identity confirmation, heavy metals screening (<20 ppb), and microbial limits (TAMC/TYMC: 0 CFU). All products are manufactured in FDA-registered facilities in the United States.

Binds to HDM-2 (MDM2) protein overexpressed in cancer cell plasma membranes (absent in normal cells); adopts p53-like HDM-2 binding conformation; forms 1:1 complexes that oligomerize into transmembrane pores (28-44 nm diameter), causing rapid membranolysis, necrosis, and cell lysis independent of p53 status or apoptosis (PNAS 2010, Biomedicines 2022) These mechanisms have been characterized in preclinical models only.

PNC-27 is supplied as a lyophilized (freeze-dried) powder and must be reconstituted before use in research applications. The standard reconstitution solvent is bacteriostatic water (sterile water containing 0.9% benzyl alcohol). When reconstituting, direct the solvent gently along the inside wall of the vial — do not spray directly onto the lyophilized powder. Allow the peptide to dissolve naturally without shaking or vortexing. For detailed reconstitution volume calculations, use the free Bacteriostatic Water Calculator or Peptide Reconstitution Calculator on Peptide.co.