5-Amino-1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]imidazole-4-carboxamide, also known as Acadesine, AICA riboside, AICAr, 5-Aminoimidazole-4-carboxamide ribonucleoside, is a small molecule research compound. CAS Number: 2627-69-2. Molecular weight: 258.23 Da.

AMPK activation in metabolism, NAFLD/fatty liver models, cancer cell proliferation/apoptosis, ischemia-reperfusion injury, inflammation (e.g., LPS-induced lung inflammation), autophagy and macrophage polarization in atherosclerosis; studies in Cells (2021) doi:10.3390/cells10051095, Toxicol In Vitro (2023), JBC (2013), BBRC (2019) All findings referenced are from preclinical and in vitro studies. No approved clinical applications exist for this research compound.

−20°C (freezer), preferably as lyophilized powder/desiccated; stable at room temp short-term but freezer for long-term Avoid repeated freeze-thaw cycles. Once reconstituted, the solution should be refrigerated at 2-8°C and used within 28 days.

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Cell-permeable nucleoside analog of AMP; enters cells via adenosine transporters, phosphorylated by adenosine kinase to ZMP (AICAR monophosphate), which binds/activates AMPK and mimics AMP effects on energy sensing pathways These mechanisms have been characterized in preclinical models only.

AICAR should be handled using standard laboratory safety protocols. Use appropriate personal protective equipment including gloves and eye protection. Work in a clean environment to prevent contamination. Follow your institution’s guidelines for handling research compounds. Refer to the Safety Data Sheet (SDS) for detailed handling instructions.